Retro-orbital sample collection in Rats - a video article

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Pharmacology, Toxicology and Biomedical Reports,2015,1,2,37-40.
Published:15th May 2015

Retro-orbital sample collection in Rats - a video article

Subramani Parasuraman*1, Khor Ming Zhen1, Ramasamy Raveendran2

1Unit of Pharmacology, Faculty of Pharmacy, AIMST University, 08100 Bedong, Kedah, Malaysia

2Department of Pharmacology, Jawaharlal Institute of Postgraduate Medical Education and Research, Pondicherry, India


Rats are the most commonly used laboratory animals for wide range of scientific research. Collection of biological fluids (such as blood, gastric fluid, breast milk and urine) is one of the important step in experiments. In many experiments, blood samples are collected for analysis of biochemical parameters and genetic profile. Blood samples are commonly collected through the tail vein, retro-orbital sinus and cardiac puncture. Retro-orbital sinus is commonly employed for collecting large volume of blood (200 μL to 1 mL) and it is employed in terminal procedures too. The main objective of this paper is to discuss the general principles of blood sample collection and the method used for orbital sinus blood sample collection.

Key words: Blood, Biological fluids, Orbital sinus, Rats, Retro-orbital.